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Figure 5


Fig. 5. Ttrap associates with Smad3 and Alk4 in mammalian cells. (A-C) Co-immunoprecipitation of TTRAP with Smad3 but not Smad2/4. HA-tagged mouse Smad2, -3 or -4 were co-produced with Flag-TTRAP (+), or Flag-TTRAP-frameshift as control (-), in HEK293T cells and precipitated using anti-Flag antibody. Precipitates were immunoblotted and co-precipitated proteins detected with anti-Smad3 or anti-HA antibody for Smad2 or Smad4. Star indicates IgG and arrow shows lack of co-immunoprecipitation with Smad2/4. (D) TTRAP-Alk4 interaction. Myc-Alk4 and Flag-TTRAP (+) or control (-) were co-produced and precipitated using anti-Flag antibody. Precipitates were analyzed with anti-Myc (top panel). Star, IgG; arrow, co-immunoprecipitation of Alk4.