Fig. 6. TH protein, phosphorylation and activity in neck-ligated flies. (A) Western blots showing TH and phospho-Ser32-TH in yw flies neck-ligated at eclosion or 30 minutes thereafter and rescue by injection of 8-Br-cAMP. (B) TH activity in the same organisms. Each value is the mean+s.e.m. of a minimum of three independent experiments.