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Figure 1


Fig. 1. Drosophila ARF6 is required for cytokinesis in testes. (A) The portion of genomic region 51f encoding Drosophila ARF6 (blue), and protein coding sequences of arf6 (red). arf61, arf62 and arf63 (below) are deletions produced by imprecise excision of EP(2)2612. (B-D) Phase-contrast images of spermatids after the second meiotic division, showing white nuclei (arrows) and black mitochondrial derivatives (Nebenkerne, arrowheads). (B) WT, (C) arf61 homozygotes: two or four nuclei per cell. (D) A rescue construct expressing arf6 from the endogenous promoter restores a 1:1 ratio of nucleus to Nebenkern per cell. Scale bars: 20 µm. (E) Frequency of nuclei per cell in WT, arf61, arf61 rescued by the arf6 endogenous rescue construct and arf63 rescued by expression of ARF6HA. n, number of cells counted.