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Figure 7


Fig. 7. Physical interaction of Pav and ARF6. (A) Growth on medium lacking histidine (-His) indicates a positive two-hybrid interaction of Pav residues 583-865, in the yeast two-hybrid screen with ARF6Q67L. pGAD, pLEX, empty vectors. (B) Pav protein domains. Five pav clones interacted with ARF6Q67L in yeast two-hybrid analysis (black bars) defining a minimum overlapping domain (ARF6 int, red). (C) Binding assay of ARF6 with Pav. Purified GSTPav655-865, but not GST alone (negative control) pulled down ARF6HA and ARF6Q67LHA from HeLa cell lysates.