Fig. 4. The Tie2 and canonical Wnt pathways are integrated through Akt. (A-E) Immunofluorescence detection of β-catenin in primary MVECs either untreated (A) or treated with Wnt3a (B), Ang1 (C), Ang1 + wortmannin (D) or Ang1 + SH6 (E). Upper and lower parts of each panel show β-catenin labeling alone and merged nuclear and β-catenin labeling, respectively. (F) Proportion of MVECS with nuclear β-catenin detectable by immunofluorescence under the conditions indicated. For this quantification, the significance levels are: ^**P<0.005, ^****P<0.00005.