Fig. 5. The angiopoietin pathway activates Wnt target genes via Akt and β-catenin. (A-L) Immunofluorescent staining for β-catenin (green and red with two rounds of labeling - see text) and nuclei (blue) in hyaloid preparations in mice of the indicated genotypes. (M,N) X-Gal staining of hyaloid vessels (red arrowheads) from TOPGAL; Lrp5^+/lacZ animals that were (M) mock injected intra-vitreally or (N) injected with Ang1 intra-vitreally. (O,P) Quantification of X-Gal-stained cell numbers (as in M and N) from hyaloid vessels of the indicated genotypes injected with Ang1 (O) or Ang2 (P). Significance levels: ^**P0.01, >0.001.