Fig. 2. Defective spermiogenesis of donor XY germ cells in recipient seminiferous tubules of XX/Sry males. (A-D) Anti-MVH (A,B) and anti-HSC70T (C,D) stainings of two serial sections of XX/Sry and XY W/W^v testes (brown staining). (C,D) Insets show higher-magnification images of HSC70T-positive spermatids: notice the abnormal morphology of all HSP70T-positive cells in XX/Sry tubules. (E-K) PAS staining (red, E-H) and transmission electron microscopic (I-K) analyses of transplanted XX/Sry and XY W/W^v testes, showing three main types of degenerating round spermatids (arrows) in recipient XX/Sry tubules: detached (E), piknotic (F,I) and large multi-nucleated giant cells (G,J). Arrowhead in G shows early elongated spermatids at steps 8-10. Asterisks indicate Sertoli cells in E-H; Sertoli cell process in I-K. (L) Quantitative analysis of transplanted XX/Sry and XY W/W^v testes showing the number of seminiferous tubules with round (steps 1-7; r-spt) or early (steps 8-10)/late (steps 11-16) elongated spermatids (e-spt) relative to the total number of tubules with spermatocytes (spc). The bar represents mean +/-standard error; asterisks indicate a statistically significant difference between the XX/Sry and XY W/W^v testes (^**P<0.01). Scale bars: 50 µmin A-H; 2 µm in I-K.