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Figure 5


Fig. 5. NADP-ICDH but not malate metabolism regulates NAD(P)H levels in the mouse oocyte. (A) Timecourse of changes in global NAD(P)H and FAD++ autofluorescence in a mature MII oocyte incubated in H-KSOM/AA after perfusion of dimethyl-malate (2-Me-malate, n=7 oocytes). Rotenone was then added to inhibit mitochondria. (B) Timecourse of changes in global (light-blue trace) and cytosolic (black trace) NAD(P)H and of global FAD++ autofluorescence (green trace) in a mature MII oocyte incubated in H-KSOM/A after sequential addition of oxalomalate and oxamate (n=25 oocytes). (C) Timecourse of changes in global NAD(P)H (blue trace) and in global FAD++ autofluorescence (green trace) after sequential addition of oxamate and oxalomalate on a mature MII oocyte incubated in H-KSOM/A (n=25 oocytes). The x-axes indicate time in minutes.