Fig. 4. E2f1 protein persists through mitosis into early S phase. (A) Stage 8 w¹¹¹⁸ embryo labeled with E2f1 (green), phospho-histone H3 (red) and phospho-tyrosine (cyan). Prophase (arrowhead), metaphase (large arrow), anaphase (small arrow), and daughter cells in early interphase (double arrow) are indicated. (B) Stage 11 w¹¹¹⁸ embryo labeled with E2f1 (green), BrdU (red; 5 minute pulse) and phospho-tyrosine (cyan). Early, mid, and late S phase are marked by a large arrow, a small arrow, and an arrowhead, respectively. Scale bars: 20 µm.