Fig. 7. Dap expression activates Rbf1. Embryos were stained for Dap (green) and phospho-tyrosine (cyan). RnrS expression was detected by FISH (red). (A) Stage 10 stg^7B/stg^7B embryo. (B) Stage 12 stg^7B/stg^7B embryo. Brackets mark the epidermal cells and asterisks denote G2₁₄-arrested aminoserosa cells. (C-E) Embryos from dap⁴⁴⁵⁴/+; stg^7B/+ parents. (C) Stage 11 embryo with the stg^7B/stg^7B phenotype. (D) Stage 11 dap⁴⁴⁵⁴/dap⁴⁴⁵⁴; stg^7B/stg^7B embryo. C and D are siblings that are stage-matched based on age, morphology and phospho-tyrosine staining. Bracket in C indicates epidermal cells in which RnrS is starting to decline and Dap is expressed at high levels. Bracket in D shows the corresponding region in which RnrS levels remain high. Asterisk in D denotes aminoserosa cells. (E) Stage 12 dap⁴⁴⁵⁴/dap⁴⁴⁵⁴; stg^7B/stg^7B embryo. Scale bar: 50 µm.