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Figure 2


Fig. 2. Wnt/ß-catenin signaling is required for zebrafish tail fin regeneration. (A) Experimental scheme. Tail fins were amputated from wild-type, hsDkk1GFP or hs{Delta}TcfGFP transgenic zebrafish and heat shocks were applied twice daily for the time periods indicated by the colored lines. (B) Continuous suppression of Wnt target gene expression in hs{Delta}TcfGFP (n=15), or reduction of Wnt/ß-catenin signaling in hsDkk1GFP transgenic fish (n=19), for 7 days starting shortly before amputation inhibits fin regeneration. Live fins were photographed at 1 dpa (left panels) and 7 dpa (right panels). (C) Overexpression of Dkk1 starting at 1 dpa inhibits fin regeneration (left panel; n=18); overexpression from 3 dpa results in partial inhibition of regeneration (right panel; 11 of 15 fins). Live fins were photographed at 7 dpa; corresponding wild-type controls regenerated normally (not shown).