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Figure 3


Fig. 3. N-cadherin mutants do not affect NC delamination. (A) Schematic of N-cadherin mutants used. (B-G) Transverse sections through embryos electroporated with: (B,D) control-GFP; (C,E) cN390{Delta}-GFP; (F) CBR-GFP; (G) JMD-GFP. (H) NT explanted on fibronectin following transfection with cN390{Delta}-GFP. (I) Quantification of NC delamination. Efficiency of electroporation was greater than 70%. Results represent the mean±s.d. of five embryos per treatment normalized to control values. The extent of NC emigration was not affected by the mutant DNAs (arrows in D-G, and I), but labeled emigrating NC cells appeared more dispersed than controls (arrows in B,C). Transfected NT cells lost epithelial morphology in cN390{Delta}-GFP and CBR-GFP-treated cases, and NC cells emigrated as round cells from NT explants (H). Scale bar: 38 µm for D-G; 45 µm for H.