Fig. 6. BMP4 stimulates N-cadherin cleavage and NC delamination via ADAM10.
Explants of neural primordia that received control medium, BMP4, GI254023X or
BMP4+GI254023X. (A,D,G,J) Phase contrast.
(B,E,H,K) GC4 anti-N-cadherin antibody that
recognizes the extracellular domain. (C,F,I,L)
N-cadherin antibody against intracellular region. (M) Quantification of
the number of mesenchymal NC cells that exited from explanted NTs
(mean±s.d. of 4-5 similar cultures per treatment). Note that whereas
BMP4 dramatically enhanced NC EMT, GI254023X inhibited both basal and
BMP-induced NC delamination and maintained full-length membrane-bound
N-cadherin under both conditions (n
12 explants per treatment
stained with each antibody). The broken white line (B,C) delineates the border
between the explant and emigrated cells. E and F exhibit only emigrated cells,
whereas H,I,K and L exhibit only densely packed but non-mesenchymal cells.
Scale bar: 100 µm in A,D,G,J; 10 µm in B,C,E,F,H,I,K,L.