Fig. 9. Marker analyses and fate mapping of tissues responding to HH signaling in Shh mutants. (A-J) Tamoxifen-induced Cre labeling with the same experimental conditions as those described in Fig. 8. (A-D) E14.5 specimens were co-stained for LacZ activity (green) and immunohistochemically stained for differentiation markers (brown). (A,B) In the bladder (but not in the GT) LacZ labeled mesenchyme co-stained with anti-smooth muscle myosin (SMM). (C,D) The LacZ-positive dorsal GT mesenchyme overlapped with the tissue expressing AR. (E-J) In Shh mutants, reduced LacZ staining was detected in the embryonic GT and bladder mesenchyme (compare E with H, and F,G with I,J). (K) A schematic depicting the distribution of HH responding tissues at E9.5-10.5 and E13.5. b, bladder; bw, body wall; c, cloaca; cm, cloacal membrane (the ventral side of the cloaca composed by inner endodermal epithelia with surface ectoderm); gt, genital tubercle; hg, hindgut; pcm, peri-cloacal mesenchyme; up, urethral plate.