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Figure 3


Fig. 3. Fate of Notch1-activated cells in neuronal lineages. (A) N1::cre labels retinal progenitors (arrows) at E14.5. (B,C) In the adult retina, clones derived from retinal progenitors that experienced Notch1 activation contain all retinal cell types in both eyes. (B) Left eye, L; (C) Right eye, R. (D) Adult cerebellum of N1::cre mice showing labeling of molecular layer (ML) and granular layer (GL) but little in the Purkinje layer (PL); note vascular staining (arrowhead) throughout. (E) {alpha}-VLLS staining (red) identifies Notch1 signaling in ventral neural tube progenitors at E11.5. (F) N1::cre marks similar progenitors at E12.5 in the ventral neural tube. (G,H) Wild-type ES cells (G) efficiently contribute to all regions of the neural tube whereas Notch1-deficient, R26 lacZ-tagged cells (H) are excluded from ventral region. Dotted line in E,G,H marks the presumptive border between neuronal progenitors and committed or differentiated motor neurons. Counter stain: Neutral Red (A-D,F), DAPI (E), Haematoxylin (G,H). FP, floor plate; DA, dorsal aorta. Magnification: A, 10x; B-H, 20x.