Fig. 7. Gene expression defects in svs mutant mice. (A-G) Real time RT-PCR comparison of mRNA levels in littermate control and svs homozygous mutant P5 seminal vesicles for genes implicated as branching morphogenesis regulators in the prostate and/or seminal vesicles. Bar graphs show the ratio of gene expression relative to 18S RNA expression; error bars show s.d. for three replicates of each measurement. Expression of Fgf10 was the same in wild-type and svs mutant mice (A). Expression of Shh (B), Gli1 (C), Gli2 (D), Ptch1 (E), Bmp4 (F) and Bmp7 (G) were reduced in svs mutant mice as compared with wild-type animals. Differences observed in B-G were statistically significant (ANOVA test with least significant difference post-hoc analysis; P value shown above each graph).