Fig. 1. Localization of Scmh1 in the adult testes and spermatocytes. (Aa) In situ hybridization using antisense probe. Stages of seminiferous tubules are given. (Ab) Higher magnification view of seminiferous tubule at stage VII shown in a. Arrows and arrowheads indicate pachytene spermatocytes and round spermatids, respectively. (Ac) Control slides using sense probe. (Ba) Immunohistochemical localization of Scmh1 of wild-type testes. (Bb) Higher magnification view of seminiferous tubule shown in a. Arrows and arrowheads indicate pachytene spermatocytes and round spermatids, respectively. (C) Immunocytochemical detection of Scmh1 gene products from zygotene to pachytene stage spermatocytes, which were prepared from day 18 pp wild-type testes. (Ca-Cd) Spermatocyte spreads were substaged into early (a) and late (b) zygotene and early (c) and late (d) pachytene stages based on anti-Scp3 (red) immunostaining and morphology. Scmh1 (green) was localized in the nuclei at each stage, but was mostly excluded from the X and Y chromosome territory at late pachytene stage, as indicated by dotted lines. (Ce) Reciprocal subnuclear localization of Scmh1 and H2A.X indicated exclusion of Scmh1 from the XY body. The XY body is indicated by dotted lines. (Cf) Reciprocal subnuclear localization of Scmh1 and dimethylated H3-K9 indicated exclusion of Scmh1 from the XY body. The XY body is indicated by dotted lines.