Fig. 2. Expression pattern of other PcG proteins and trimethylated H3-K27 at pachytene stage spermatocytes. (A) Immunocytochemical detection of Prc1 components in pachytene spermatocytes. (Aa,Ab) Phc1 (a) and Phc2 (b) were excluded from the XY body demarcated by extensive accumulation of H2A.X. (Ac-Ae) Bmi1 (c), Rnf110 (d) and Cbx2 (e) were excluded from the XY body demarcated by extensive accumulation of uH2A. The XY body is indicated by dotted lines. (Af) Reciprocal subnuclear localization of Phc2 and dimethylated H3-K9 indicated exclusion of Scmh1 from the XY body. (B) Immunocytochemical detection of trimethylated H3-K27 from late zygotene to pachytene stage spermatocytes. Spermatocytes were immunostained by using anti-trimethylated H3-K27 (red) and anti-SCP3 (green). The X and Y chromosome territory is indicated by dotted lines.