Fig. 3. Testicular abnormalities in Scmh1^-/- mice. (A) Cross-sections of testes from day 35 pp wild-type (Aa) and Scmh1^-/- (Ab) mice. Sections were stained with Hematoxylin and Eosin (HE). (B) The frequency of Scmh1^-/- mice in which seminiferous tubules were morphologically affected during first-wave spermatogenesis. Days after birth are shown. At each age, more than ten mutants were examined. Mutants over 8 weeks of age were collected and indicated as adults (Ad). (C) Increased apoptotic spermatocytes in Scmh1^-/- testes. (Ca-Cc) Incidence of apoptosis in wild-type testes at day 7, 15 and 19 pp. (Cd-Cf) Incidence of apoptosis in Scmh1^-/- testes at day 7, 15 and 19 pp. (Cg,Ch) Higher magnification views of individual seminiferous tubules shown in e and f. Outline of seminiferous tubules are indicated by dotted lines. (D) The expression of stage-specific markers during spermatogenesis in wild-type and unaffected and affected Scmh1^-/- testes at day 35 pp, as revealed by semi-quantitative RT-PCR. ß-actin was used as a standard to verify the equal amounts of cDNA. Primers used in each reaction are shown in Table 1. Scale bars: 100 µm in A,B,Ca-Cf; 10 µm in Cg,Ch.