Fig. 5. Altered chromatin modifications at the XY body in Scmh1^-/- spermatocytes. (A) Immunostaining for dimethylated H3-K9, monomethylated H3-K9, phosphorylated RNA pol II, H2A.X and uH2A in the spermatocyte spread. (Aa,Ab) Frequency of spermatocytes, in which dimethylated (a) or monomethylated (b) H3-K9 was enriched at the XY body demarcated by uH2A accumulation, was compared (left) and results were summarized (right). (Ac,Ad) Frequency of spermatocytes, in which phosphorylated RNA pol II (c) or acetylated H3-K9 (d) were excluded from the XY body, was compared (left) and results were summarized (right). (Ba,Bb) Frequency of spermatocytes, in which Phc1 (a) or Phc2 (b) were excluded from the XY body, was compared (left) and results were summarized (right). (Bc,Bd) Frequency of spermatocytes, in which trimethylated H3-K27 were excluded from the XY body was compared. Scp3 was used to substage the spermatocytes. (C) Frequency of spermatocytes, in which monomethylated H3-K4 (Ca) and dimethylated H4-K20 (Cb) were accumulated on the XY body, was compared (left) and results were summarized (right).