Fig. 4. N activity specifically influences Pros expression in LG. All panels show three segments of a stage 15-16 VNC with axons (anti-HRP) shown in green in A,C,G,H. All other antibodies are as labeled. htl-Gal4 was used to drive expression of UAS transgenes. (A) Misexpression of Hairless (H) abolishes nearly all Pros expression in LG, while Pros in neurons is unaffected (arrowhead). (B) Overlay of Pros in A with anti-GFP reveals that H does not alter the number or positioning of LG. (C) Misexpression of Numb also abolishes Pros expression. (D,E) In wild type, the enzyme Gs2 is restricted to the six anterior LG. (F) In contrast to Pros, Gs2 expression in the anterior LG is unaffected by misexpression of Numb. (G) Misexpression of N^ICD causes Pros expression in most or all LG, but does not alter the number or position of LG (H). (I) Overlay of G and H without anti-HRP and with Pros converted to green. (J) Quantification of Pros-positive LG per hemisegment: misexpression studies. Quantification performed as in Fig. 2J, with results binned as shown in the legend.