Fig. 1. Impacts of hexamerin silencing on downstream gene expression. Downstream effects of hexamerin silencing (A) on the expression of 17 putative downstream genes, and the three reference/control genes ß-actin, NADH-dh and HSP-70 (B). Results were determined from quantitative real-time PCR data. See Table 1 for gene abbreviations. The y-axis represents the percentage of gene expression relative to water-injected controls 24 hours after treatment (bars represent the mean of five individuals, each determined in triplicate). Bars with asterisks represent significant differences from water-injected controls at P<0.01 (^***), P<0.05 (^**) or P<0.1 (^*), obtained by pairwise Kruskal-Wallis tests. Error bars represent standard error of the mean (s.e.m.).