Fig. 2. Temporal expression changes for 22 genes in R. flavipes workers across days 5, 10 and 15 of isolation experiments. (A,B) Isolation away from the colony (A) and in the presence of 150 µg externally-applied JH III (B). Results are normalized to colony workers at day 0 (shown by arrows at top of A and B). (C) JH III-treatment effects normalized to colony-release effects within each day (shown by arrows in the right portion of C). Yellow and blue cells, respectively, indicate significant increases and decreases in gene expression (P<0.05), whereas black cells indicate no change (P>0.05). Statistical analyses were made using the Kruskal-Wallis test from three independent replications, each determined in triplicate. See Table 1 for gene abbreviations. Values presented in individual cells are means, with standard error (s.e.m.) shown in parentheses.