Fig. 3. Comparison of gene expression by linear regression analysis. Linear regressions comparing the expression of 17 genes between hexamerin silencing experiments and experiments involving (top row) isolation away from the colony, and (bottom row) isolation on JH III deposits at days 5 (left), 10 (middle) and 15 (right) of isolation. Correlation coefficients and P-values were determined using PROC REG in the SAS software package. Colony-release results shown across the top row indicate no significant correlation with hexamerin silencing, whereas JH-dependent results in the bottom row show significant correlations with hexamerin silencing for all days (P<0.05). Also, all JH regressions in the bottom row improve after the removal of the apparent outlier genes apoptosis inhibitor, ATPase, larval cuticle protein, nanos and SH3 kinase (compare full and reduced models). See Fig. 1 and Fig. 2A,C for original data presentations and standard error (s.e.m.) estimates that correspond to each data point.