Fig. 9. Fly, chick and mouse gcm genes induce expression of neuronal and glial markers in HeLa cells. GFAP (A,B,C,D), GFP (A',B',C',D'), Tuj1 (A'',B'',C'',D'') and DAPI (A''',B''',C''',D''') colabeling and merges (A'''',B'''',C'''',D'''') of HeLa cells transfected with expression vectors carrying c-Gcm1 (A-A''''), gcm (d-gcm1 in B-B''''), mouse Gcm1 (m-gcm1 in C-C'''') or vector as control (D-D''''). Scale bar: 20 µm. (E) Percentage of transfected cells expressing neuronal/glial markers. n indicates the number of GFP-positive cells counted. Note that all gcm1 genes induce the expression of GFAP and Tuj1 markers and that these two markers colocalize in most transfected cells.