Fig. 2. Mouse planar cell polarity phenotypes. (A) Fully open neural tube (craniorrhachischisis) in a Fz3^-/-;Fz6^-/- fetus at embryonic day (E) 18. (B) Open eyelids in a Fz3^-/-;Fz6^-/- fetus at E18, shown by phalloidin staining of the anterior two-thirds of the eye; the edges of the eyelids are indicated by white arrows. (C) Hair follicle orientation defects on the dorsal surface of the Fz6^-/- paw at postnatal day (P) 8. Proximal (left) and distal (right). The bases of the central digits are immediately beyond the right edge of each image. (D) Defects in inner ear sensory hair bundle orientation at E18 in a Vangl2 mutant (Lp/Lp), organ of Corti flat mount. Top, actin-rich stereocilia are labeled with phalloidin (red) and kinocilia are labeled with anti-acetylated tubulin (green). Bottom, diagrams of hair bundle orientations for each image. IHC, inner hair cells; OHC1, inner row of outer hair cells; OHC2, central row of outer hair cells; OHC3, outer row of outer hair cells. Reproduced with permission from Wang et al. (Wang et al., 2006b).