Fig. 2. The reciprocal effects of ectopic expression or loss of Slp and Iro. (A-A'') mirr-lacZ (red) is repressed by ectopic slp (green, arrow). Inset in A'' is higher magnification showing the autonomous repression of mirr-lacZ (arrows mark diminishing staining inside dorsal slp). (B-B'') slp-lacZ (red) is ectopically expressed in the inner regions of iro mutant clone (loss of green). Note that the ectopic slp-lacZ evident dorsal to the arrowed clone is an outgrowth of eye tissue into the head capsule. Why slp is non-autonomously induced in such outgrowths is unclear. (C-C'') slp-lacZ (red) is repressed by ectopic mirr (green, arrow). Inset in C'' shows the non-autonomous effects on slp-lacZ. Immediately surrounding the clone staining is absent, and surrounding this is a ring of cells showing decreased expression. (A''', B''', C''') Schematic summaries of the effects observed.