Fig. 2. Role of targeted AUX1 expression in lateral root formation.
(A) Root tip of a GAL4-GFP-expressing line (J0951) used for targeted
expression of AUX1 to the lateral root cap and epidermis. Arrowheads
mark the end of the root cap. (B,C) Analysis of lateral root
initiation and positioning in wild type (Col-0), aux1-22, both
parental lines (UAS:AUX1,aux1-22 and J0951,aux1-22), and
transactivation line (J0951>>AUX1,aux1-22). (B) Lateral root
density (seedlings grown at 45°). *, statistically significant
differences for values compared with wild type as determined by Student's
t-test (P<0.001). (C) Quantification of left-right
alternation of lateral roots in vertically grown seedlings. *,
statistically significant difference for right side values compared with wild
type as determined by Student's t-test (P<0.001). Error
bars, s.e.m.