Fig. 5. AXR3 dependency for asymmetric cell division in the xylem pole pericycle. (A) J0121 with specific expression in the xylem pole pericycle starting from the basal meristem onward. (B-E) Analysis of lateral root formation in wild-type control (Col-0), the parental lines (J0121 and UAS:axr3-1), and the transactivation line (J0121>>UAS:axr3-1). (B) Lateral root densities. ^*, statistically significant differences for values compared with wild type as determined by Student's t-test (P<0.001). (C) Lateral root stages [total number of detected primordia from ten individual roots at each developmental stage according to Malamy and Benfey (Malamy and Benfey, 1997)]. (D) Differential interference contrast image of adjacent pericycle cells with migrated nuclei in J0121>>UAS:axr3-1. ^*, nuclei; arrowhead, cell wall. (E) Average number of lateral roots per seedling at stage I-VIII (gray) and arrested at the stage of displaced nuclei (white). ^*, statistically significant difference for values for displaced nuclei as determined by Student's t-test (P<0.02). Error bars, s.e.m.