Fig. 1. sel-2 loss of function enhances lin-12(d) activity in VPCs. (A) Late L4 stage N2 (left) and sel-2(n655) lin-12(n302) (right) hermaphrodites. Top panels show Nomarski photomicrographs, with the arrow(s) indicating a normal vulva (N2) or pseudovulval invaginations; bottom panels show clusters of cells expressing egl-17::cfp-lacZ (arIs92). (B) Schematic view of the predicted sel-2 operon, indicating the regions used for rescue and RNAi experiments. (C) The sel-2 genomic region and a sel-2 translational reporter `rescue' the enhancement of lin-12(n302) by sel-2(n655). Arrays are described in Materials and methods. Multivulva (Muv) is defined as 3 pseudovulvae. The number of worms scored is indicated above each bar. (D) sel-2(RNAi) enhances lin-12(d). pFV denotes the empty RNAi feeding vector. Except for mab-21(RNAi), each bar represents the mean of at least two independent experiments; error bars are the s.e.m. Muv is defined as 2 pseudovulvae; a minimum of 100 animals was scored for each treatment.