Fig. 7. Accumulation of FM4-64 in the intestine after injection into the pseudocoelom (basolateral delivery). (A) FM4-64 fluorescence in sel-2(+) and sel-2(ar219). (B) FM4-64 fluorescence (middle panel, red) is shown relative to autofluorescent granules (left panel, green); co-localized signals are shown in yellow in the merge (right panel). Arrows indicate irregularly shaped, FM4-64 labelled compartments that are not coincident with autofluorescence in sel-2(ar219). (C) Quantification of the `sel-2' mutant phenotype (see text). Rescue of the phenotype with an extrachromosomal array carrying the sel-2 genomic region and phenocopy with sel-2(RNAi) is also shown. All scoring was done blind to genotype. Each bar is the mean of at least two independent experiments, and error bars are the s.e.m. The total number of worms scored is indicated on each bar.