Fig. 7. Continuous Ovhts expression is necessary for Ovhts-RC and actin localization to RCs. (A) Expression of P{UASH-Ovhts::GFP} with MTD-Gal4 results in expression of Ovhts::GFP during all stages of oogenesis and continuous localization to RCs. (B) Expression of P{UASH-Ovhts-GFP} with the P{bam-GAL4} driver in wild-type ovaries shows expression and localization of Ovhts::GFP to RCs in the germaria and faintly in stage 2 (arrowhead). There is no Ovhts::GFP protein on RCs at later stages that are actinpositive (arrow). (C-D) Expression of P{UASH-Ovhts::GFP} in hts^G with P{nos-Gal4}. The P{nos-Gal4} driver produces Ovhts::GFP expression (green) in early and late stages, but no expression in intermediate stages. Staining with Rhodamine-conjugated phalloidin shows that F-actin localization to RCs mirrors Ovhts::GFP expression. (D) Higher magnification of an intermediate stage egg chamber that is not expressing Ovhts::GFP. F-actin is in clumps that may be degenerating RCs. Scale bars: A,B, 20 µm; C,D, 10 µm.