Fig. 3. Fgfr2^cn prostates exhibited basic prostate characteristics. (A) Prostate tissues from 6-week-old mice were sectioned and stained with HE for histological analyses. Inserts: high-magnification views from the same tissues. (B) Total RNAs were extracted from dorsolateral prostates of 3-week-old mice and reverse translated with random hexanucleotide primers. RT-PCR was performed as indicated, with ß-actin and Gapdh as internal standards. Cycle numbers of amplification are indicated at the top. (C) Real-time RT-PCR analyses of the same panel of molecules as in B. Data were normalized with ß-actin loading control and were expressed as folds of difference from the control prostates. Data were means of triplicate samples. ^*P<0.001. F/F, homozygous Fgfr2^flox mice; CN, Fgfr2^cn mice; ap, anterior prostate; dp, dorsal prostate; lp, lateral prostate; vp, ventral prostate.