Fig. 3. Stable zic2aD5:gfp expression recapitulates endogenous midbrain and posterior hindbrain expression. Gfp expression in Tg(zic2aD5:gfp) embryos detected by ISH (A,D,G,H), or by fluorescence (B,E). (C,F,I) Endogenous zic2a expression detected by ISH. (A-C) gfp RNA at the 11- to 12-somite stage (A) and Gfp protein at 14-somites (B) are restricted to dorsal midbrain and posterior hindbrain. Endogenous zic2a is transcribed in the dorsal diencephalon, midbrain and hindbrain at 11-somites (C). (F-H) Transgenic (D,E) and endogenous (F) expression overlap in the midbrain and posterior hindbrain at the 20-somite stage. At 24 hpf, transgenic expression (G,H) overlaps endogenous expression (I) in the dorsal midbrain. All views are lateral, anterior to the left, except H,I which are dorsal views. Arrowheads mark anterior and posterior limits of tectum. m, midbrain; h, hindbrain.