Fig. 1. Deletion alleles of mrg-1. (A) The mrg-1 gene structure with exons (boxes), introns (joining lines), and the 3' UTR (gray). Black bars below the gene indicate regions deleted by each allele. (B) Wild-type and mrg-1(qa6200) M-Z-embryos co-stained with rabbit anti-MRG-1 antibody (red) and DAPI (blue). Scale bar: 10 µm. (C) Western blot analysis of 100 N2, mrg-1(tm1227) and mrg-1(ok1262) M+Z-adult hermaphrodites reacted with rabbit anti-MRG-1 (upper panel) and mouse anti-tubulin as a loading control (lower panel).