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Figure 4


Fig. 4. MRG-1 associates with autosomes independently of the MES proteins. (A-D) One-cell wild-type embryo at pronuclear meeting doubly stained with mouse PA3 to stain DNA (A) and rabbit anti-MES-4 (C) and imaged for GFP::MRG-1 (B). (D) Merged image of DNA (red) and GFP::MRG-1 (green). Arrows point to chromatin lacking both MRG-1 and MES-4. (E-M) One-cell embryos at pronuclear meeting stained with mouse PA3 (E,H,K and red in merge) and rabbit anti-MRG-1 (F,I,L and green in merge). (E-G) Wild-type embryo. (H-J) mes-2(bn76)rol-1 M-Z-embryo. (K-M) mes-4(bn73) M-Z-embryo. Arrows point to presumptive X chromosomes. (N-S) One-cell embryos at pronuclear meeting stained with mouse PA3 (N,Q and red in merge) and rabbit anti-MES-4 (O,R and green in merge). (N-P) Wild-type embryo. (Q-S) mrg-1(qa6200) M-Z-embryo. Arrows point to X chromosomes. Scale bars: 10 µm.