Fig. 6. MRG-1 is not required for activation of transcription of at least some germline genes in PGCs. (A) RT-PCR comparison of mRNA levels in wild-type and mrg-1(qa6200) M-Z-L1 larvae. All transcript levels were normalized to ama-1 mRNA. Histograms show mean±s.e. of three independent experiments. (B-D) Expression of a paternally contributed pgl-1(+) allele in pgl-1/+ heterozygous L1s derived from mating pgl-1/pgl-1 mutant hermaphrodites, untreated (B) or treated with mrg-1 RNAi (C,D), with wild-type males. L1s were stained with anti-PGL-1. (E,F) Appearance of newly synthesized GLH-1 in wild-type and mrg-1 M-Z-L1s, seen by staining with anti-GLH-1 (green). PA3 stain of DNA is in red. Values in the upper right corners are numbers of L1s that displayed that staining pattern. Scale bar: 10 µm.