Fig. 7. MRG-1 is required for several mep-1 mutant phenotypes. (A-F) L1 larvae stained with DAPI (blue) and anti-PGL-1 (red) (A-C) or anti-GLH-1 (red) (D-F). mep-1(RNAi) larvae displayed ectopic expression of PGL-1 and GLH-1 in somatic cells (B,E). Ectopic expression was reduced in mep-1(RNAi); mrg-1(RNAi) larvae (C,F). Arrowheads point to the position of PGCs. Scale bar: 50 µm. (G) RT-PCR comparison of pgl-1, glh-1, nos-1 and myo-2 mRNA levels in L1 and L2 larvae with the genotype indicated. ama-1 was used as the internal reference. Histograms show mean±s.e. of four independent experiments. (H) Percent of larvae that developed into adults after treatment of their mothers with the RNAi reagents shown. RNAi of mep-1 induced larval arrest. RNAi of mrg-1 but not dpy-5 strongly suppressed the mep-1(RNAi) larval arrest phenotype.