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Figure 6


Fig. 6. SRF binds FAST-1. (A) GST-tagged SRF was transfected into 293T cells together with Myc-FAST-1. Cell lysates were pulled down by glutathione-agarose beads and then immunoblotted with anti-Myc antibody. (B) Schematic representation of the structure of FAST-1. FKHD, Forkhead DNA-binding domain. Myc-tagged FAST-1 deletion mutants were transfected into 293T cells together with GST-SRF. Cell lysates were pulled down by glutathione-agarose beads and then immunoblotted with anti-Myc antibody.