Fig. 6. SRF binds FAST-1. (A) GST-tagged SRF was transfected into
293T cells together with Myc-FAST-1. Cell lysates were pulled down by
glutathione-agarose beads and then immunoblotted with anti-Myc antibody.
(B) Schematic representation of the structure of FAST-1. FKHD, Forkhead
DNA-binding domain. Myc-tagged FAST-1 deletion mutants were transfected into
293T cells together with GST-SRF. Cell lysates were pulled down by
glutathione-agarose beads and then immunoblotted with anti-Myc antibody.