Fig. 6. Interaction between PCP genotype and RhoA-ROCK signalling. Percent embryos with closed neural tube (i.e. successful Closure 1; upward bars) or open neural tube (i.e. failure of Closure 1; downward bars) after 18 hours culture from E7.5. Data plotted against concentration of ROCK inhibitor, Y27632, or JNK inhibitor, SP600125. Hatched bars represent +/+ embryos. Solid bars represent Lp/+ (A,B), Crsh/+ (C) and Crc/+ (D) embryos. While Y27632 affects Closure 1 in wild-type embryos only at the highest concentration, and not at all in circletail litters, heterozygous embryos of all three strains show failure of Closure 1 at lower Y27632 concentrations. Neurulation is not adversely affected by SP600125 in +/+ or Lp/+ embryos. For number of embryos and statistical analysis, see Table 2.