Fig. 2. Notch2-deficient kidneys (N2) develop distal tubules without formation of podocytes or proximal tubules. (A,B) Wild-type (A) kidney contains cells expressing high levels of Wt1 in glomerular podocytes and S-shaped bodies. The only cells that express low levels of Wt1 in the mutant (B) are mesenchymal cells surrounding cytokeratin-8-expressing ureteric buds (red). (C,D) LTL-stained proximal tubules found in wild type (D) are absent in the mutant (C). (E,F) Mutant kidney (E) develops numerous E-cadherin-positive, cytokeratin-8-negative distal tubules, some of which are connected to cytokeratin-8-positive ducts (dashed line in enlarged view E'). The wild-type proximal tubules, judged by morphology, also express E-cadherin (arrowhead in F). N2, Notch2 mutant; Wt, wild type. Scale bars: 0.1 mm in B for A,B,E'; in C for C,D.