Fig. 3. Notch2-deficient mesenchyme undergoes normal epithelialization but the newly formed nephron fails to resolve into the S-shaped body seen in wild type. (A-E) Wild type (A) showing S-shaped bodies (arrowheads). In the Notch2 mutant, one nephron, expressing N-Cam, can be identified at each ureteric bud tip (arrowheads in B). These synthesize laminin 1 (arrowheads in D), as do the S-shaped bodies (arrowheads in C). (E) Three progressive stages during nephrogenesis in the mutant are marked as 1, 2 and 3. (F-J) Expression of molecular markers in each of the three segments in the S-shaped body (see text). Distal tubule precursors are Pax2^High- and E-cadherin-positive (F,H). Jag1 is localized in the middle segment in the position of proximal tubule precursors (G). Wt1 marks the podocyte precursors (I). N1-ICD is detectable in both the proximal and podocyte precursors (J). Scale bars: 0.05 mm in A for A-D and in J for F-J; 0.1 mm in E.