Fig. 3. NC derivatives of lateral, but not medial, migration pathway are defective in cho mutants. (A,B) Alcian Blue staining of craniofacial cartilage in 5 dpf WT (A) and cho mutant (B) embryos. (C-H) Anti-Hu detection of enteric neurons at 3 dpf in WT (C) and cho mutant (D) embryos, and of DRG sensory neurons at 48 hpf (E,F) and 5 dpf (G,H) in WT (E,G) and cho mutant (F,H) embryos. As reported previously in WT embryos (An et al., 2002), we observed some ectopic sensory neurons at both 48 hpf and 5 dpf (arrows, F,H; see also Table 1). (I-P) In situ hybridisation with GTP-cyclohydrolase (gch) on WT (I,K,M,O) and cho mutants (J,L,N,P) at 24 hpf (I,J), 30 hpf (K,L), 36 hpf (M,N) and 48 hpf (O,P). (Q,R) 48 hpf. cho mutant collar xanthophore defects are independent of melanophores. In situ hybridisation with gch showed that whilst nac mutants had a WT xanthoblast pattern throughout the trunk (Q), cho;nac double mutants still displayed loss of xanthoblasts from the collar region (R). Embryos are shown in lateral view, anterior to the left; except for A,B which are ventral views, anterior up. Scale bars: A,B, 50 µm; C,D,G,H, 75 µm; E,F, 100 µm; I-P, 150 µm; Q,R, 175 µm.