Fig. 8. Abnormal primordium migration results in misrouting of the PLL nerve in anterior-most trunk of cho mutant embryos. (A,B) Whole-mount mRNA in situ hybridisation of cxcr4b in 34 hpf WT (A) and cho mutant (B) to show aberrant migration of PLL primordium (arrow). (C,D) This results in misrouting and truncation of PLL nerve (arrow), as seen by zn-12 immunohistochemistry on 56 hpf WT (C) and cho mutant (D) embryos. Somites are numbered. Scale bars: 50 µm in A,B; 20 µm in C,D.