Fig. 2. Supernumerary pharynx formation after Smed-roboA RNAi. (A,B) Confocal projections showing ectopic pharynges after 16 days of regeneration and two rounds of RNAi treatment. Anti-phosphotyrosine and anti-VC-1 labeling is shown. In A, arrows indicate pharyngeal opening; in B, arrows indicate connections between ectopic and pre-existing gut. (C,D) laminin (DN293829) in situ hybridization in 16-day regenerates. (C) Control; (D) Smed-roboA RNAi. In all panels, white asterisks indicate original pharynges; magenta asterisks show ectopic pharynges. Anterior is to the left. cg, cephalic ganglia; g, ectopic gut branches. Scale bars: 200 µm in A; 100 µm in B; 300 µm in C,D.