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Figure 4


Fig. 4. Phylogenetic analysis and expression domains of Lhx6 and Lhx7 in Astyanax. (A) Lhx6 and Lhx7 sequences from different species were aligned and a phylogenetic tree was constructed. (B,C) Forty-eight hpf in toto lateral views of Lhx6 (B) and Lhx7 (C) expression patterns (purple) in cavefish brains that have been double-hybridised with Shh (red). (D,E) At 36 hpf, in toto lateral (D) and ventral (E, high power) views of cavefish brains double-hybridised for Lhx6 (purple) and Lhx7 (red). Scale bars: 50 µm. fp, floor plate; hyp, hypothalamus; pit, pituitary; po, preoptic region; SP, subpallium; zli, zona limitans.