Fig. 6. Overexpression of sema3D causes ectopic left habenular projections. (A-F) Lateral views of the 4-day-old larval IPN from (A,D) mock-injected embryos (n=91) or embryos injected with (B,E) sema3D mRNA (n=64), or (C,F) sema3Gb mRNA (n=69). Larvae were stained with anti-Lov antibody (red) and anti-Ron antibody (green) in A-C, or labeled with DiI (red) in the left habenula in D-F. (G,H) Lateral views of (G) 2- and (H) 4-day-old larvae double-labeled with sema3D antisense RNA probe and anti-Lov antibody. sema3d transcripts (red) are abundant in the brain region dorsal to the IPN, as visualized by Lov⁺ habenular projections (green). Scale bars: 50 µm.