Fig. 3. catp-1 modulates C. elegans L2 developmental rate.
(A) catp-1(kr17) specifically delays the timing of L2/L3 molt.
During the lethargus period that precedes molting, rythmic contractions of the
pharynx (also called pharyngeal pumping) ceases. Each dot represents the
percentage of worms pumping at a given time (n>25 individuals).
(B) catp-1(kr17) is insensitive to DMPP-induced developmental
delay. L2 development was divided into five stages based on seam cell
(encircled nuclei) divisions and anchor cell differentiation
(Ruaud and Bessereau, 2006).
Developmental stage was monitored using DIC optics, which did not allow the
discrimination between classes 3 and 4. The proportion of worms belonging to
each class was scored 32 hours after egg laying (N
12
individuals). Error bar represents s.e.m., n=3 independent
experiments.