Fig. 2. Cks30A, Cort and Fzy regulate overall cyclin levels in the egg. Western blots of eggs aged 0-2 hours from unfertilized (females crossed to X0 males) wild type (yw), Cks30A^KO/Cks30A^KO (cks), cort^QW55/cort^RH65 (cort), fzy⁶/fzy⁷ (fzy), and cort^QW55,fzy⁶/cort^RH65,fzy⁷ (cort,fzy) kept at 29°C and probed for cyclin B3, cyclin A and cyclin B. Cdk1, a stable cell-cycle regulator, serves as a loading control. Cyclin B is slightly elevated in Cks30A, more so in fzy and cort, and is highly elevated in fzy; cort double mutants. Cyclin A and cyclin B3 are both elevated in cort and fzy, but elevation is higher in cks30A and fzy; cort double mutants.