Fig. 3. Cort can mediate the destruction of mitotic regulators and its activity depends on Cks30A. (A-C') UAS-HA-Cort expressed under ptcGal4 and detected with anti-HA antibodies. Cyclin A (A'), cyclin B (B') and cyclin B3 (C') are reduced in the stripe, corresponding to Cort expression. (D,D') UAS-Fzy expressed under ptcGal4 (detected with anti-ß-gal antibodies; D) results in a corresponding reduction in cyclin A levels (D'). (E-H) Magnified area of an adult wing from Drosophila expressing HA-Cort (E,G,H) or UAS-Fzy (F) in the posteriorhalf of the wing (P) using enGal4. The anterior-half of the wing (A) serves as a control. Expression of UAS-HA-Cort (E) or UAS-Fzy (F) in the posterior wing results in a wider spacing of wing hairs. (G) Wing-hair-spacing phenotype is suppressed when HA-Cort is expressed in a Cks30A^KO/Cks30A^KO background. (H) Co-expression of UAS-Flag-Cks30A with HA-Cort in a Cks30A^KO/Cks30A^KO background restores the wing-hair-spacing phenotype. (I) Expression of Flag-Cks30A alone does not affect bristle spacing. Scale bar: 20 µm.